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Cores
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The UCSF Sandler Center Functional Genomics Core Facility was established in 2000 and facilitates the use of microarray technology by providing state-of-the-art technology and technical expertise. We offer a comprehensive and integrated approach to array analysis. Our multidisciplinary group interacts closely with investigators and offers support for all phases of each study, including experimental design, sample preparation, hybridization, quality control, data analysis, and database maintenance. Projects funded by the Sandler Program for Asthma Research are given special priority and a substantial discount.
In 2006, our core converted from 'home-made' arrays to the Agilent 60mer ink-jet platform. We have very extensive experience with using the arrays for mRNA expression and in the past year we have developed, optimized, and validated miRNA arrays that have probes for miRNAs from many species including human, mouse, and rat. We can analyze expression in small samples (<1 ng total RNA for mRNA analysis and ~100 ng total RNA for miRNA analysis). Arrays are available in multiple formats and are easily customizable at no additional cost. We can also provide access to other platforms, including Affymetrix GeneChips, through established collaborations with other cores at UCSF.
Information about our core is available at http://www.arrays.ucsf.edu.
David Erle, MD, Professor of Medicine, core director, david.erle@ucsf.edu, 415-514-4370
Andrea Barczak, associate director, andrea.barczak@ucsf.edu, 415-514-4373
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